Anti-Ki-67 Antibody

Raised in rabbit and validated for human samples, this unconjugated polyclonal reagent from Antibodies.com targets the Ki-67 nuclear proliferation antigen, a non-histone protein expressed during the G1, S, G2, and M phases of the cell cycle but absent in resting G0 cells.

The antibody detects the human MKI67 gene product and is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, packaged in a 100 µl vial. It is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications, making it suitable for proliferation studies, tumor grading, and cell-cycle analysis in cancer research.

Ki-67 is widely used by pathologists as a marker of actively dividing cells, particularly in breast carcinoma, neuroendocrine tumors, and lymphoma subtyping, where the proliferative index informs prognosis and treatment decisions. Storage at -20 °C is recommended, and the inclusion of glycerol as a cryoprotectant allows the antibody to remain stable through repeated freeze-thaw cycles when aliquoted.