Anti-Keratin 5 Antibody

This rabbit polyclonal antibody targets the type II cytokeratin known as Keratin 5 (KRT5), a 58 kDa intermediate filament protein characteristic of basal cells in stratified and pseudostratified epithelia. Antibodies.com provides this unconjugated reagent at 100 µL per vial, raised in rabbit and affinity-purified for use in Western blot and immunohistochemistry workflows on human, mouse, and rat samples. KRT5 is widely studied as a basal-layer marker in skin, oral mucosa, esophagus, and mammary gland tissue, and it is frequently used to define the basal-like molecular subtype of triple-negative breast cancer in tumor pathology research. The antibody recognizes the native filament protein as well as denatured forms, supporting both chromogenic IHC on formalin-fixed paraffin-embedded sections and immunoblotting of epithelial cell lysates. Researchers investigating epidermal differentiation disorders, squamous cell carcinoma progression, and epithelial stem cell compartments routinely rely on anti-KRT5 reagents to distinguish proliferating basal keratinocytes from their differentiated suprabasal counterparts, which instead express Keratin 1 and Keratin 10. The unconjugated format allows users to couple the primary antibody to their preferred fluorophore or enzyme label depending on the detection platform.