Anti-Keratin 20 Antibody

This rabbit polyclonal reagent from Antibodies.com targets Keratin 20, a type I cytokeratin expressed in the gastrointestinal epithelium, urothelium, and Merkel cells. The unconjugated antibody is supplied as 100 µl of liquid and is validated for Western blot and immunohistochemistry workflows. Reactivity has been confirmed against human, mouse, and rat samples. In immunohistochemistry, KRT20 staining is frequently applied by pathology labs to support the diagnosis of colorectal, gastric, pancreatic, and urothelial adenocarcinomas, and to help identify Merkel cell carcinoma in cutaneous biopsies. The polyclonal format recognizes multiple epitopes along the keratin intermediate filament, which can be useful in formalin-fixed paraffin-embedded tissue where epitope masking is common. For Western blot, the antibody is suited to detection of the roughly 48 kDa keratin 20 band in lysates from epithelial cell lines or tumor tissue. Refrigerated storage is recommended for the working aliquot; researchers can freeze the stock for longer term use and limit freeze-thaw cycles. Each lot is quality controlled as a primary antibody for epithelial lineage studies, tumor marker profiling, and investigations of gastrointestinal or urothelial differentiation.