Anti-Keratin 18 Antibody

Keratin 18 (KRT18) is a type I intermediate filament protein expressed in simple and glandular epithelia, where it contributes to cytoskeletal integrity and serves as a marker in epithelial and apoptosis research. This unconjugated Mouse monoclonal IgG2a reagent targets the KRT18 polypeptide and is validated for Western blot (WB) and immunocytochemistry (ICC) workflows on Human and Monkey samples. The antibody is supplied by Antibodies.com as 100 µl of ready-to-titrate reagent, sufficient for multiple blot and staining experiments depending on working dilution. Because intact K18 is cleaved by executioner caspases during programmed cell death, antibodies against the full-length protein are routinely used alongside M30 neo-epitope reagents to distinguish stages of epithelial apoptosis in cell culture and tissue models. The unconjugated format gives laboratories flexibility to couple the antibody to HRP, biotin, or fluorophores according to their detection chemistry, supporting both chemiluminescent Western blot and fluorescent ICC protocols.