Anti-KDR (phospho Y1214) Antibody

Phospho-specific detection of VEGFR-2 signaling is enabled by this polyclonal reagent raised against KDR phosphorylated at tyrosine 1214, a site implicated in VEGF-driven endothelial proliferation and vascular permeability responses. Antibodies.com supplies this affinity-purified rabbit antibody in unconjugated IgG format, intended for Western blot analysis of human samples. Each vial contains 100 µl of reagent, with reactivity verified against KDR-expressing human cell lines and primary endothelial cultures. Researchers investigating angiogenic pathways, tumor vasculature, or VEGFR2 downstream signaling can apply the antibody to lysates prepared under conditions that preserve the Y1214 phosphorylation state, then probe membranes with appropriate secondary reagents. Storage at -20 °C in working aliquots helps maintain phospho-epitope recognition across multiple experiments, while avoiding repeated freeze-thaw cycles preserves antibody activity. The reagent pairs with phospho-Y1175 VEGFR-2 antibodies for studies that compare distinct receptor phosphorylation events, supporting dissection of VEGF-A, VEGF-C, and VEGF-E mediated activation across in vitro endothelial model systems.