Anti-KDM5A Antibody [18E8]

The Mouse monoclonal 18E8 antibody from Antibodies.com recognizes KDM5A (Lysine Demethylase 5A), also referred to as JARID1A or RBP2, a Jumonji C domain-containing histone demethylase that removes methyl groups from H3K4me3 and H3K4me2. Produced as an IgG2a isotype in unconjugated liquid format, this reagent has been validated for western blot, flow cytometry, and immunofluorescence workflows in human and mouse samples. Supplied at 100 µg per vial, the antibody has been applied in chromatin and transcriptional regulation studies, including research into its role as a transcriptional co-repressor in leukemia and solid tumor contexts. For western blot, lysates prepared in RIPA buffer typically yield a band near the expected 196 kDa molecular weight of full-length KDM5A. Aliquot upon receipt and store frozen at -20 °C to preserve activity; brief storage at 4 °C is acceptable for short-term use, but repeated freeze-thaw cycles should be avoided.