Anti-KCTD15 Antibody

This primary polyclonal antibody from Antibodies.com targets the BTB/POZ domain protein KCTD15, a non-channel regulator that physically binds the activation domain of AP-2α (TFAP2A) and represses AP-2-driven transcription at the neural plate border. The reagent is supplied as 50 µl of unconjugated rabbit IgG antiserum, validated for western blot, immunohistochemistry, and immunofluorescence on human, mouse, and rat samples. Documented application dilutions include WB at 1:1000 on cell and tissue lysates and IHC at 1:100 on paraffin-embedded sections, with IF on cultured cells and fixed tissue also supported. The unconjugated format allows flexible pairing with fluorophore- or HRP-tagged secondary reagents chosen by the user. For storage, the vial should be aliquoted and stored at -20 °C; avoid repeated freeze-thaw cycles to preserve antibody activity. Research uses include mapping KCTD15 expression in neural crest specification, examining its role in Wnt/β-catenin signaling and NF-κB pathway modulation, evaluating its function in adipogenesis and obesity genetics (GWAS-linked to BMI), and tracking its expression in melanoma cell biology and acute leukemia biomarker studies.