Anti-KCNJ1 Antibody

Raised in rabbit against the KCNJ1 inward-rectifier potassium channel, this unconjugated polyclonal IgG primary antibody from Antibodies.com is supplied as 50 µl of ready-to-use reagent for western blot and immunohistochemistry workflows. KCNJ1, also called ROMK (Renal Outer Medullary Potassium channel), is an ATP-sensitive inward rectifier on the apical membrane of renal tubular epithelial cells, where it recycles potassium into the tubular lumen and supports sodium reabsorption through the Na-K-2Cl cotransporter. Validated reactivity with mouse and rat tissue makes the antibody a practical choice for rodent models of renal physiology, channel trafficking studies, and investigations into Bartter syndrome type II, a hereditary tubulopathy caused by loss-of-function mutations in KCNJ1. As an unconjugated primary reagent, it leaves the choice of secondary detection open, from HRP-based chromogenic staining on fixed kidney sections to fluorescence imaging of membrane fractions. Researchers commonly pair it with ROMK literature references when probing thick ascending limb and collecting duct preparations. The vial should be kept refrigerated, briefly centrifuged before opening to recover full volume, and protected from light; aliquot on first use to limit freeze-thaw cycles.