Anti-KCNC3 Antibody

Raised in rabbit against a synthetic peptide mapping near the C-terminus of the human KCNC3 (Kv3.3) channel, this unconjugated polyclonal IgG reagent from Antibodies.com recognises endogenous levels of the voltage-gated potassium channel subunit in human, mouse, and rat samples. The 100 µl affinity-purified antibody is supplied in PBS (pH 7.3) with 0.05% sodium azide and 50% glycerol; it is shipped at 4 °C and should be aliquoted and stored at -20 °C, avoiding repeated freeze/thaw cycles. Recommended for western blot analysis of total protein lysates, the reagent supports studies of cerebellar Purkinje cell excitability, parvalbumin-positive cortical interneurons, auditory brainstem nuclei, and the molecular pathology of spinocerebellar ataxia type 13 (SCA13). Representative validation data show an 8% SDS-PAGE blot of 293T cells and mouse brain lysates at 40 µg per lane using a 1:300 primary dilution, with HRP-conjugated secondary at 1:8000 and a 1-minute ECL exposure.