Anti-KCNC2 Antibody

Raised in rabbit against a synthetic peptide corresponding to residues near the N-terminal of human KCNC2, this polyclonal IgG reagent from Antibodies.com is antigen affinity purified and validated for Western blot detection of endogenous Kv3.2 protein. The antibody cross-reacts with mouse and rat orthologues and has been quality-tested on an 8% SDS-PAGE gel using 50 µg per lane of 231 cells and human brain malignant glioma tissue lysate at a working dilution of 1:450, with an HRP secondary at 1:8000 and a 10-second exposure. Supplied as 50 µl of liquid at 0.9 mg/mL in PBS (pH 7.3) with 0.05% sodium azide and 50% glycerol cryoprotectant, the vial is shipped at 4 °C and should be aliquoted for storage at -20 °C to limit freeze-thaw cycles across repeated blots. The reagent targets the Shaw-related voltage-gated potassium channel subunit implicated in fast-spiking GABAergic interneurons of the neocortex, hippocampus, and caudate nucleus, and supports immunoblotting studies of neuronal excitability, ion channel regulation, and neurological disease models such as epilepsy and cerebellar ataxia. Researchers can probe brain, spinal cord, or cultured neuron lysates to resolve total KCNC2 around its predicted ~62 kDa band.