Anti-Jun (phospho T91) Antibody

This phospho-specific reagent from Antibodies.com is a rabbit polyclonal antibody that selectively recognizes c-Jun when phosphorylated at threonine 91, a regulatory modification linked to downstream modulation of AP-1 transcriptional activity. Supplied as 50 µl of unconjugated IgG in a single-use vial, the antibody is validated for Western blot detection of endogenous pJun (T91) levels in human cell and tissue lysates. It is raised in rabbit, is of the IgG isotype, and is supplied unconjugated so it can be paired with HRP-, fluorophore-, or biotin-conjugated secondary reagents to suit chemiluminescent or fluorescent immunoblotting workflows.

Typical research uses include time-course studies of upstream kinase activation following stress or mitogenic stimulation (UV exposure, cytokines, growth factor withdrawal), comparison of basal versus induced pJun (T91) levels across treatment groups or genetic backgrounds, and characterization of signaling events involved in apoptosis, proliferation, and differentiation programs. For best preservation of the phospho-epitope, store at -20°C in small aliquots and avoid repeated freeze-thaw cycles; refrigerated storage of working dilutions is acceptable. For research use only.