Anti-JUN (phospho S243) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against the phosphorylated serine 243 residue of human c-Jun, a core component of the AP-1 transcription factor complex. Phosphorylation at S243 is a regulatory event linked to the DNA damage response, oxidative stress signaling, and cell cycle progression, making this reagent useful for probing JUN-dependent transcriptional control in research settings.

The antibody is supplied in a 50 µl volume and is unconjugated, giving researchers flexibility to pair it with secondary detection reagents of their choice. It is validated for Western blot (WB) applications and is reactive with human JUN. As a polyclonal IgG reagent raised in rabbit, it offers broader epitope coverage than monoclonal alternatives, an advantage when studying post-translational modifications that can be sensitive to local conformational context around the phospho-epitope.

Typical uses include monitoring JUN S243 phosphorylation kinetics following genotoxic insult, tracking stress-activated kinase cascades, and comparing basal versus induced phosphorylation states across treated and untreated cell lysates. For best results, store at -20 °C and divide into single-use aliquots to limit repeated freeze-thaw cycles, which can erode phospho-specific binding activity over time.