Anti-JNK1/2/3 (phospho-Y185) Antibody

Raised in rabbit and supplied by Antibodies.com as an unconjugated polyclonal reagent, this antibody recognizes the phosphorylated tyrosine 185 site shared by JNK1, JNK2, and JNK3 stress-activated kinases. The product is formulated at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, and each vial contains 100 µl of ready-to-use solution.

Validated for western blot and immunohistochemistry on human, mouse, and rat samples, the reagent supports phospho-specific detection of c-Jun N-terminal kinase activation in signaling pathway studies. Investigators probing the MAPK cascade in response to cytokines, osmotic shock, UV exposure, or oxidative stress rely on the Y185 phospho-epitope as a marker of JNK enzymatic activity. The polyclonal format captures multiple determinants around the phospho-site, helping to maintain signal consistency across membrane and tissue-based assays. Store refrigerated and protect from light to preserve the activity of the sodium azide-preserved solution.