Anti-JNK1/2/3 Antibody

JNK1, JNK2, and JNK3 are stress-activated serine/threonine kinases encoded by MAPK8, MAPK9, and MAPK10 that phosphorylate c-Jun and other transcription factors, transducing signals triggered by cytokines, UV exposure, and osmotic shock. This unconjugated recombinant rabbit monoclonal antibody from Antibodies.com was raised against a shared epitope and recognises all three isoforms, supporting studies of MAPK signalling in apoptosis, inflammation, and neuronal stress response.

Validated applications include Western blot and immunocytochemistry/immunofluorescence staining, enabling assessment of total JNK protein levels and subcellular localisation in cultured cells and tissue sections. Reactivity has been confirmed across human, mouse, rat, cow, monkey, and zebrafish samples, allowing use in comparative and translational research models.

The reagent is supplied as 50 µl of recombinant rabbit monoclonal antibody in a vial, formulated in 1×TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative. For long-term storage, aliquot and store at -20°C, protecting the solution from repeated freeze-thaw cycles. Suggested starting dilutions are 1:500–1:2000 for Western blot and 1:50–1:200 for ICC/IF, with empirical titration recommended for each experimental system.