Anti-JAK3 (Ab-785) Antibody

JAK3 (Janus Kinase 3) is a non-receptor tyrosine kinase essential for signal transduction through the common gamma chain cytokine receptor family, governing lymphocyte development, proliferation, and immune cell function. This polyclonal reagent from Antibodies.com is raised in rabbit and selectively detects human JAK3 phosphorylated at tyrosine 785, a regulatory site implicated in kinase activation downstream of cytokine receptor engagement.

The antibody is supplied as 100 µl of unconjugated antisera, validated in western blot and immunohistochemistry workflows against human samples. Its polyclonal format increases the likelihood of recognizing JAK3 even when the phospho-Tyr785 epitope is partially masked, while the unconjugated format lets researchers pair it with HRP, biotin, or fluorophore-conjugated secondary reagents. For best results, aliquot upon arrival and store at -20°C, avoiding repeated freeze-thaw cycles to preserve antibody activity.

Because JAK3 expression is largely restricted to hematopoietic lineages, phospho-JAK3 (Tyr785) serves as a useful marker in studies of T-cell receptor signaling, NK cell biology, and primary immunodeficiency research, including severe combined immunodeficiency (SCID) caused by JAK3 loss-of-function mutations. The reagent is validated for detection of the activated kinase in cell lysates and fixed tissue sections.