Anti-Jak2 (Ab-1007) Antibody

Supplied by Antibodies.com as a primary antibody, this reagent is a rabbit polyclonal antibody raised against JAK2, with the immunogen corresponding to a peptide sequence around amino acids 1005–1009 (K-E-Y-Y-K) of the human JAK2 protein. The unconjugated antibody is reactive with Human, Mouse and Rat samples and is validated for Western blotting (WB).

Antibodies were produced by immunising rabbits with the synthetic peptide coupled to KLH and purified by affinity chromatography using the epitope-specific peptide. The antibody detects the endogenous level of total JAK2 protein. The product is supplied at a concentration of 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol.

For Western blotting, the manufacturer recommends a working dilution of 1:500–1:1000. The predicted molecular weight of JAK2 is 125 kDa. The antibody is shipped at 4°C and, upon delivery, should be aliquoted and stored at -20°C; repeated freeze/thaw cycles should be avoided.