Anti-Jagged1 Antibody

Jagged1 (JAG1) is a single-pass transmembrane ligand that activates Notch receptors on adjacent cells, and this rabbit polyclonal reagent from Antibodies.com is raised against it for western blot and ELISA detection. The antibody is supplied as 100 µl of unconjugated serum, reactive with the human, mouse, and rat proteins.

Engagement of JAG1 with NOTCH1-4 triggers sequential proteolytic cleavages that release the Notch intracellular domain, allowing it to enter the nucleus and modulate CSL/RBP-Jκ-dependent transcription. Researchers apply such antibodies to track Jagged1 expression during angiogenesis, hematopoietic stem cell maintenance, T-cell lineage commitment, vascular remodeling, and tumor-stroma crosstalk where Notch signaling is frequently dysregulated. Because polyclonal reagents recognize multiple epitopes on the Jagged1 extracellular region, they can accommodate minor sequence variation between species.

In Alagille syndrome, JAG1 haploinsufficiency drives multi-organ developmental defects, and this type of antibody is used to assess expression in patient-derived material and corresponding animal models. Aliquot on arrival and store at the temperature recommended on the vial to preserve binding activity over the working life of the product.