Anti-ITGB5 Antibody

From Antibodies.com, this affinity-purified rabbit polyclonal reagent is raised against a recombinant human ITGB5 (integrin beta-5) immunogen and is designed to detect the endogenous level of total ITGB5 protein across multiple species. The antibody is supplied as an unconjugated liquid at a concentration of 1.0 mg/mL in a 50 µL volume, buffered in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. Validated applications include Western blot (WB) and immunohistochemistry (IHC), with demonstrated performance on paraffin-embedded human kidney sections at a 1:200 dilution and on Western blots of extracts from various cell lines. The reagent shows confirmed reactivity with human, mouse, and rat samples, making it a versatile option for cross-species integrin research, including studies of cell adhesion, signalling through αvβ5, and tumour-cell biology. Polyclonal formatting provides recognition of multiple epitopes on the ITGB5 subunit, supporting robust signal in both denaturing (WB) and fixed-tissue (IHC) workflows. For storage, the recommended condition is -20 °C (or lower for long-term), with aliquots to avoid repeated freeze–thaw cycles, and the product is shipped on wet or dry ice from St. Louis, MO with a lead time of 6–9 business days. Intended for research use only, this Antibodies.com primary antibody is suited to laboratories investigating integrin-mediated pathways, cytoskeletal remodelling, and related downstream signalling events, and is also available in a larger 100 µL format and via custom bulk requests for higher-throughput studies.