Anti-ISL1 Antibody

This polyclonal antibody from Antibodies.com targets ISL1 (ISL LIM homeobox 1), a LIM-homeodomain transcription factor with central roles in cardiac lineage specification, pancreatic islet cell development, and motor neuron differentiation. The reagent is supplied as 100 µl of unconjugated rabbit antiserum at 1 mg/ml in PBS with 0.1% Sodium Azide and 50% glycerol, a cryoprotective formulation that supports storage at -20 °C and resistance to repeated freeze-thaw cycles. The antibody has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples, supporting studies that trace ISL1 expression in embryonic cardiomyocyte progenitors, dorsal root ganglia, and pancreatic islet tissue. Researchers using this reagent commonly examine ISL1-dependent transcriptional programs, including its co-regulatory interactions with LHX3 and downstream activation of cardiac genes such as NKX2-5 and MEF2C. The polyclonal nature of the reagent allows recognition of multiple epitopes on the ISL1 protein, which can improve signal in tissues where target expression is low, such as the sinoatrial node and the developing enteric nervous system.