Anti-IRS-1 (Y632) Antibody

Raised in rabbit and affinity-purified, this unconjugated polyclonal antiserum targets a phosphorylated tyrosine residue (Y632) within the IRS-1 adaptor protein, a central node in insulin and IGF-1 receptor signaling. Tyrosine 632 lies within a YXXM motif that, once phosphorylated, recruits the p85 regulatory subunit of PI3-kinase to drive downstream Akt/PKB signaling cascades relevant to glucose uptake, growth, and survival.

Provided at 1 mg/ml in phosphate buffered saline (PBS), pH approximately 7.2, with 0.05% sodium azide as a preservative, the reagent is packaged as a 50 µl vial that supports multiple immunohistochemistry runs on formalin-fixed, paraffin-embedded tissue sections. Antibodies.com confirms reactivity with human, mouse, and rat samples, making this antibody useful in translational research on insulin resistance, type 2 diabetes, metabolic syndrome, and IRS-1 dysregulation in tumor tissue, where it can help localize phospho-IRS-1 distribution relative to insulin receptor-positive cell populations. For storage, the sodium azide-containing PBS formulation is intended for refrigerated handling to preserve antibody activity over long-term use.