Anti-IRS-1 (V317) Antibody

The IRS-1 (Insulin Receptor Substrate 1) protein is a key cytoplasmic adaptor that mediates downstream signaling from the insulin and IGF-1 receptors. This rabbit polyclonal antibody from Antibodies.com is raised against a synthetic peptide centered on the V317 residue of human IRS-1, a target widely used to study insulin resistance, metabolic disease, and growth-factor signaling pathways in research use only applications.

Validated for immunohistochemistry (IHC) and immunofluorescence (IF), the reagent recognizes IRS-1 in human, mouse, and rat samples. The unconjugated IgG is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and the 50 µl vial size supports multiple staining runs in standard immunohistochemistry protocols.

Because IRS-1 functions as a docking protein for PI3K and other downstream effectors, antibodies against defined epitopes are commonly applied to map activation states in tissue contexts. The polyclonal format detects the target across a range of fixation conditions, making this reagent useful in both paraffin-embedded sections and freshly prepared cryosections when probing phosphorylation-dependent events in insulin signaling research.