Anti-IRS-1 (R301) Antibody

Rabbit polyclonal antibody targeting insulin receptor substrate-1 (IRS-1), raised against a synthetic peptide corresponding to the R301 region of the human protein. From Antibodies.com, this unconjugated primary reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and is provided as a 50 µl aliquot sufficient for multiple immunoblots.

The antibody recognizes IRS-1 in human, mouse, and rat samples and has been validated across western blot, immunohistochemistry, and immunofluorescence workflows. Its polyclonal coverage of the R301 epitope makes it a practical tool for researchers studying insulin and IGF-1 receptor signalling, since IRS-1 is a key cytoplasmic substrate of the insulin receptor tyrosine kinase and a central node in downstream PI3K-Akt signalling relevant to metabolic disease, cancer biology, and in vitro cell-signalling studies.

As an unconjugated reagent, it pairs with any standard secondary detection system the researcher selects (HRP, fluorophore, or biotin), allowing flexibility across PVDF or nitrocellulose membrane exposures, formalin-fixed paraffin-embedded tissue sections, and immunofluorescence imaging of cultured cells. The product should be stored refrigerated and protected from light; for long-term use, small aliquots should be frozen to limit repeated freeze-thaw cycles of the parent vial.