Anti-IRS-1 (R301) Antibody

Insulin Receptor Substrate-1 (IRS-1) is a central cytoplasmic adaptor that couples the activated insulin and IGF-1 receptors to downstream PI3K/Akt signaling, and the Antibodies.com Anti-IRS-1 (R301) polyclonal antibody was raised against a defined epitope within the protein's phosphotyrosine binding (PTB) domain — the docking region through which IRS-1 engages the insulin receptor and becomes tyrosine phosphorylated.

The reagent is produced in rabbit and purified as an unconjugated polyclonal antiserum, supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2. Validated applications include Western blot, immunohistochemistry, and immunofluorescence on human, mouse, and rat samples, making it broadly useful across metabolic, neuroscience, and oncology research models that interrogate insulin and growth-factor pathways. Because it is supplied unconjugated, investigators can pair it with their preferred HRP, biotin, or fluorophore-conjugated secondary detection reagent.

Each vial contains 100 µl of polyclonal antibody. For long-term stability, store at -20°C in single-use aliquots and avoid repeated freeze-thaw cycles; the included 0.05% sodium azide acts as a preservative for working dilutions kept refrigerated short term. The antibody is well suited to studies of insulin resistance, neuronal insulin signaling, and IRS-1 phosphorylation dynamics in cancer metabolism.