Anti-IRS-1 (phospho-S794) Antibody

Phosphorylation of insulin receptor substrate 1 at serine 794 (IRS-1 pS794) is a regulatory event driven largely by mTORC1 and its downstream kinase S6K1, providing a mechanistic readout of negative feedback on insulin and IGF-1 signaling. This unconjugated rabbit polyclonal antibody from Antibodies.com enables immunohistochemistry-based detection of that phospho-epitope in tissue sections, supporting studies of insulin resistance, metabolic disease models, and oncogenic mTOR pathway activation.

The reagent is supplied at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, packaged as a 100 µl vial. It recognizes IRS-1 in human, mouse, and rat samples, making it compatible with the most commonly used preclinical model systems. Standard IHC workflows on formalin-fixed paraffin-embedded tissue are supported, and the polyclonal format offers multiple epitope contacts around the S794 phospho-site, which can be useful when accessibility of the modified residue varies between preparations.

For optimal signal, process tissues with antigen retrieval appropriate for phospho-epitopes (such as citrate or EDTA heat retrieval) and detect with an HRP- or fluorescence-conjugated secondary reagent. Endogenous phosphatase activity should be considered during fixation; adding phosphatase inhibitors or using rapid cold fixation helps preserve the S794 phosphorylation mark. Aliquot the antibody to limit repeated freeze-thaw cycles and refrigerate to maintain activity across multiple uses.