Anti-IRS-1 (phospho-S612) Antibody

Phospho-specific detection of IRS-1 (Insulin Receptor Substrate 1) at serine 612 is enabled by this polyclonal antibody from Antibodies.com, raised in rabbit and supplied as an unconjugated reagent at approximately 1 mg/ml. The antibody selectively recognizes the phosphorylated S612 epitope, a site associated with insulin and IGF-1 receptor signaling dynamics relevant to metabolic disease and cell growth studies.

Validated for Western blot and immunohistochemistry, the reagent supports detection of phospho-S612 IRS-1 across human, mouse, and rat samples, enabling cross-species comparison in signaling pathway research. Each 100 µl vial is formulated in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, and should be stored at -20 °C with aliquots prepared to limit freeze-thaw cycles. The unconjugated format allows flexible pairing with secondary antibodies labeled with HRP, fluorophores, or biotin for diverse detection chemistries in PI3K/Akt and downstream insulin signaling investigations.