Anti-IRS-1 (phospho-S323) Antibody

Raised in rabbit against a phosphopeptide surrounding serine 323 of human IRS-1, this polyclonal antibody from Antibodies.com selectively detects the phosphorylated form of Insulin Receptor Substrate 1. IRS-1 is a cytoplasmic adaptor protein that couples the activated insulin and IGF-1 receptors to downstream PI3K/Akt signaling, and phosphorylation at S323 has been characterized as a regulatory event linked to insulin resistance and metabolic signaling studies.

The reagent is supplied as 100 µl of a 1 mg/ml stock in PBS containing 0.1% sodium azide and 50% glycerol, in an unconjugated format that pairs readily with a user-selected secondary antibody. It has been validated for Western blot, immunohistochemistry, and immunofluorescence workflows, with reactivity confirmed against human, mouse, and rat samples.

Typical applications include probing insulin-stimulated cell lysates, mapping IRS-1 phosphorylation dynamics in fixed tissue sections, and imaging the subcellular distribution of phosphorylated IRS-1 in cultured cells. The polyclonal nature of the reagent provides broader epitope coverage around the phospho-S323 site, which can be useful when investigating kinase-dependent modifications in the insulin signaling cascade. For handling, store refrigerated and protect from light, avoiding repeated freeze-thaw cycles to preserve binding activity across extended experiments.