Anti-IRS-1 (phospho-S307) Antibody

From Antibodies.com, this rabbit polyclonal reagent detects IRS-1 only when phosphorylated at serine 307, a modification that marks insulin signaling attenuation. The antibody is provided as 100 µl of liquid at 1 mg/ml in phosphate buffered saline containing 15 mM sodium azide, with pH near 7.2. The unconjugated format supports chromogenic, fluorescent, or chemiluminescent detection in western blot, immunohistochemistry, and immunofluorescence workflows.

The reagent cross-reacts with IRS-1 from human, mouse, and rat origin, enabling comparative studies across cell culture and tissue models. Serine 307 becomes phosphorylated by stress-activated kinases such as JNK, IKKβ, mTOR, and PKCθ, so this antibody is commonly used to monitor insulin resistance in type 2 diabetes, obesity, and metabolic syndrome research. Additional applications include cancer biology, where altered IRS-1 phosphorylation influences tumor growth, and neuroscience studies of brain insulin signaling.

As a polyclonal, the antibody binds several epitopes surrounding the phospho-S307 site, supporting consistent detection of dynamic phosphorylation changes after stimulation with insulin, cytokines, or small-molecule kinase inhibitors. Store refrigerated and avoid repeated freeze-thaw cycles to maintain activity; aliquoting upon first use is recommended for long-term projects.