Anti-IRS-1 (P618) Antibody

Rabbit polyclonal antiserum raised against a defined region of Insulin Receptor Substrate 1 (IRS-1), a cytoplasmic adaptor protein that couples activated insulin and IGF-1 receptors to downstream PI3K/Akt and MAPK signaling cascades. This unconjugated reagent is validated for immunohistochemistry (IHC) on formalin-fixed tissue sections and cross-reacts with human, mouse, and rat samples, supporting translational studies of metabolic regulation, insulin resistance, and growth factor signaling.

The antibody is supplied as 100 µl of purified Ig at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, ready to dilute into standard IHC workflows. Refrigerated handling preserves the 1 mg/ml formulation for repeated use across staining runs. Because IRS-1 sits at the hub of insulin and IGF-1 signal transduction, this reagent is frequently cited in research on type 2 diabetes, obesity, hepatic glucose output, neuronal insulin signaling, and cancer cell proliferation pathways.

For laboratories comparing insulin-sensitive versus insulin-resistant phenotypes, pairing this antibody with downstream readouts such as phospho-Akt or phospho-ERK staining helps localize where IRS-1 signaling diverges in disease models. Antibodies.com provides this reagent as part of its primary antibody portfolio for academic and translational research use.