Anti-IRS-1 (Ab-636) Antibody

Rabbit polyclonal IgG raised against a synthetic peptide spanning residue region 636 of human Insulin Receptor Substrate 1 (IRS-1), supplied by Antibodies.com as a 100 µl aliquot of unconjugated serum-derived reagent. This antibody recognizes the endogenous IRS-1 adaptor protein in western blot, immunohistochemistry, and immunofluorescence workflows, with reactivity validated for human, mouse, and rat samples. IRS-1 functions as a critical cytoplasmic substrate of the insulin and IGF-1 receptor tyrosine kinases, relaying signals from activated receptors to downstream PI3K-Akt and MAPK cascades that govern glucose uptake, mitogenic responses, and metabolic homeostasis. Researchers in diabetes, neuroendocrinology, and growth-factor signaling use this reagent to monitor tyrosine phosphorylation events, total expression, and tissue localization in cultured cells and fixed tissue sections. For best results, aliquot upon receipt and store frozen, limiting freeze-thaw cycles to preserve epitope recognition across downstream applications.