Anti-IRS-1 (Ab-312) Antibody

Manufactured and supplied through Antibodies.com, this rabbit polyclonal reagent targets Insulin Receptor Substrate 1 (IRS-1) at the serine 312 region and is offered in a 50 µl format at a protein concentration of 1.0 mg/mL. The antibody was raised against a synthesized non-phosphopeptide derived from human IRS-1 surrounding the phosphorylation site serine 312 (A-T-S(p)-P-A), allowing it to detect endogenous levels of total IRS-1 protein rather than the phosphorylated form alone. It is unconjugated and consists of Rabbit IgG formulated in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using the epitope-specific immunogen, ensuring high specificity for the target. Validated applications on the supplier datasheet include Western Blotting (WB), Immunohistochemistry (IHC), and Immunocytochemistry/Immunofluorescence (ICC/IF), and the reagent has been confirmed to react with both human and mouse samples. Shipping is performed at 4 °C, and upon receipt the product should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Suited to research laboratories studying insulin signaling, metabolic regulation, and receptor tyrosine kinase downstream pathways, this polyclonal antibody provides a reliable tool for monitoring total IRS-1 expression across a range of cell- and tissue-based assays.