Anti-IRS-1 (Ab-312) Antibody

Produced as a rabbit polyclonal reagent, this unconjugated primary antibody from Antibodies.com binds IRS-1, an insulin receptor substrate adapter protein that couples the activated insulin and IGF-1 receptors to downstream PI3K/Akt and MAPK signaling cascades. The Ab-312 designation defines a specific epitope within the target, and the polyclonal format recognizes multiple epitopes on the same molecule, supporting reliable detection across varied sample preparations.

Validated applications include Western blot for analyzing IRS-1 expression in cell and tissue lysates, immunohistochemistry on tissue sections for localization studies, and immunofluorescence for subcellular distribution in cultured cells. Reported reactivity covers human and mouse samples, enabling comparative studies between model systems and clinical material.

Supplied as 100 µl of unconjugated antiserum, the reagent can be paired with secondary reporters of choice — HRP enzymes, fluorophores, or biotin-streptavidin systems — to match specific assay requirements. Working aliquots are recommended to limit repeated freeze-thaw cycles of the stock vial.