Anti-IRF-3 Antibody

The polyclonal reagent from Antibodies.com is raised against Interferon Regulatory Factor 3 (IRF-3), a transcription factor central to the innate antiviral immune response. Upon viral detection by cytosolic pattern recognition receptors, IRF-3 is phosphorylated by TBK1 and IKKε kinases, dimerizes, translocates to the nucleus, and cooperates with CBP/p300 to drive expression of type I interferons and downstream interferon-stimulated genes.

This unconjugated rabbit polyclonal antibody is validated for Western blot, immunohistochemistry, and ELISA workflows and recognizes IRF-3 in human, mouse, and rat samples, supporting comparative studies in infectious disease, inflammation, and cancer biology models. Researchers apply it to track endogenous IRF-3 expression and phosphorylation-dependent mobility shifts in cell lysates, and to localize IRF-3-positive nuclei in formalin-fixed tissue sections.

Supplied as a 100 µl vial, the reagent fits routinely into signaling pathway analyses, viral pathogenesis investigations, vaccine adjuvant development, and tumor immunology projects where type I IFN responses are of interest. The polyclonal format broadens epitope coverage across IRF-3 isoforms and post-translational modifications commonly examined in innate immunity research.