Anti-IRAK2 Antibody

From Antibodies.com, this rabbit polyclonal antibody targets Interleukin-1 Receptor-Associated Kinase 2 (IRAK2), a serine/threonine kinase that functions downstream of Toll-like receptors and the IL-1 receptor in innate immune signaling pathways. The unconjugated reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, in a 100 µl vial, and is validated for Western blot and immunohistochemistry on human, mouse, and rat samples.

IRAK2 has drawn sustained research interest because it operates both as an active kinase and as a scaffold in TLR-mediated NF-κB and MAPK activation, and because altered IRAK2 protein levels have been documented in chronic lymphocytic leukemia B cells, diffuse large B-cell lymphoma, and several solid tumor models. Laboratories studying inflammation, cytokine signaling, tumor-microenvironment cross-talk, and innate immune tolerance commonly use anti-IRAK2 reagents to confirm knockdown efficiency, map signaling intermediates downstream of MyD88, and compare protein expression between disease and normal tissue by IHC.

Store at -20°C in working aliquots to limit freeze-thaw cycles; the 100 µl vial supports multiple experimental batches. Working dilutions should be titrated empirically for Western blot on whole-cell lysates and for IHC on formalin-fixed paraffin-embedded sections, and reactivity should be confirmed on the user's own sample types because IRAK2 levels vary across immune cells, epithelial lines, and primary tissue.