Anti-INTS5 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com recognizes Integrator Complex Subunit 5 (INTS5), a 908-amino-acid component of the multiprotein Integrator complex that associates with the C-terminal domain of RNA polymerase II and mediates 3'-end processing of U1 and U2 snRNAs in eukaryotic cells. The unconjugated primary antibody is supplied in a 200 µl vial and is validated for Western blot detection of human and mouse INTS5, with reactivity confirmed against whole-cell lysates such as MCF7, HT29, and mouse liver. The immunogen corresponds to amino acids 120-400 of the human INTS5 sequence, an internal region shared across expressed isoforms, and the reagent performs reliably under standard reducing SDS-PAGE conditions where INTS5 resolves near its predicted molecular weight of approximately 107 kDa (observed around 108-120 kDa depending on gel system). Recommended western blot working dilution is 1:500-1:2,000, with chemiluminescent detection following HRP-conjugated secondary reagents; signals have been demonstrated from as little as 25 µg of total protein per lane. Researchers studying snRNA biogenesis, transcription termination, or Integrator complex assembly can use this antibody to probe expression patterns, validate knockdown or knockout efficiency, and co-detect INTS5 alongside other Integrator subunits. The polyclonal format provides epitope diversity that is well suited to detecting the full-length endogenous protein in cell and tissue extracts. Storage at -20°C with standard freeze-thaw handling preserves activity across the product's 12-month shelf life. Intended for research use only.