Anti-Intestinal-type alkaline phosphatase Antibody

Generated in rabbit as an unconjugated polyclonal reagent, the Antibodies.com primary antibody recognizes the human intestinal-type alkaline phosphatase (IAP) isoform, the brush-border membrane-bound enzyme expressed by mature enterocytes of the small intestine. Each vial contains 100 µg of affinity-purified IgG and is validated for western blot and immunohistochemistry applications, supporting both protein-level detection in cell and tissue lysates and localization studies on formalin-fixed, paraffin-embedded sections. Researchers investigating mucosal differentiation, intestinal epithelial barrier integrity, gut inflammation models, and alkaline phosphatase isoform biology use this reagent to distinguish the intestinal variant from tissue-nonspecific placental, hepatic, and bone isoforms through differential band patterns and tissue-restricted staining. Because the preparation is unconjugated, end users can match it with HRP, biotin, or fluorescent secondary reagents of their own choice, making it straightforward to drop the antibody into existing colorimetric or immunofluorescence pipelines. For best results, store refrigerated in single-use aliquots to limit freeze-thaw cycles and preserve binding activity over multi-week experiments.