Anti-Interferon gamma Antibody [315]

Clone 315 is a Mouse monoclonal IgG1 antibody produced by Antibodies.com for selective detection of human Interferon gamma, a 21-25 kDa homodimeric cytokine central to Th1-type cell-mediated immunity. The unconjugated reagent ships as a 100 µg lyophilised vial, allowing extended storage and reconstitution into working aliquots.

Validated for ELISA and Western blot workflows, the reagent helps quantify IFN-γ secretion in lymphocyte culture supernatants and identify the cytokine band in lysed cell preparations. Because it is unconjugated, researchers can pair it with their preferred secondary reporter—HRP, biotin, or fluorophore conjugates—depending on the detection platform.

Typical applications include profiling Th1/Th2 balance in autoimmune disease studies, characterizing T cell effector function in vaccine research, and tracking NK cell activity in tumor immunology. Reconstitute the lyophilised pellet in sterile buffer, aliquot to limit freeze-thaw cycles, and store refrigerated for routine use; freeze working stocks below -20°C for long-term retention of binding activity. Each preparation from Antibodies.com is accompanied by lot documentation to support reproducible experimental setup.