Anti-Integrin α4 Antibody

Raised in rabbit against Integrin α4 (also known as ITGA4 or CD49d), this unconjugated polyclonal antibody from Antibodies.com is supplied as 100 µl of ready-to-use reagent validated for Western blot and ELISA workflows. The antibody recognizes the α4 integrin subunit in samples from human, mouse, and rat, supporting comparative immunology studies across species.

Integrin α4 pairs with β1 to form VLA-4 and with β7 to generate the α4β7 heterodimer; both complexes mediate leukocyte rolling, firm adhesion, and transendothelial migration during immune surveillance and inflammatory responses. Researchers studying lymphocyte trafficking, hematopoietic stem cell homing, or autoimmune disease models (including multiple sclerosis and inflammatory bowel disease, where α4 integrins are established therapeutic targets) frequently use this reagent to probe α4 expression in lymphoid tissues and primary cell isolates.

Polyclonal preparations recognize multiple epitopes on the target, providing robust signal across denaturing conditions used in Western blot analysis. Each lot is validated for specificity, and the unconjugated format allows flexible pairing with user-selected secondary reporters. The reagent should be refrigerated; avoid repeated freeze-thaw cycles by aliquoting upon first use. For research use only.