Anti-Integrin β5 Antibody

The polyclonal reagent from Antibodies.com recognizes Integrin α5 (ITGA5), the α-subunit of the α5β1 heterodimer that serves as the principal fibronectin receptor on most cell types. Raised in rabbit and supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated antibody is validated for Western blot (WB) and immunohistochemistry (IHC) on human, mouse, and rat samples, supporting studies of cell–extracellular matrix adhesion, angiogenesis, fibronectin signaling, and tumor cell migration. The polyclonal format allows multi-epitope recognition, useful when probing endogenous ITGA5 levels in tissue sections or whole-cell lysates where post-translational modifications or splice variation may mask individual epitopes. Researchers in cancer biology, vascular biology, and developmental signaling rely on α5β1 pathway readouts to investigate wound healing, metastatic behavior, and integrin-mediated mechanotransduction. Each vial is provided ready-to-use after brief centrifugation, and storage at -20 °C in single-use aliquots preserves activity across freeze-thaw cycles. The azide-containing buffer is compatible with standard HRP- and fluorophore-conjugated secondary reagents for both chromogenic and fluorescence detection workflows.