Anti-Insulin Antibody

Polyclonal reagent raised in guinea pig hosts provides sensitive detection of insulin in immunoassay workflows. Produced as neat serum and supplied in a 1 ml vial, this unconjugated primary antibody from Antibodies.com is optimized for ELISA-based quantification of insulin in research samples. Guinea pig immunoglobulins offer an alternative host species for studies where mouse or rabbit-derived reagents may produce background concerns.

The antibody targets endogenous insulin, supporting investigations into pancreatic beta-cell function, glucose homeostasis, and diabetes pathophysiology. Researchers employ this reagent in ELISA protocols to measure insulin secretion from cultured islets, cell culture supernatants, or tissue extracts. As an unconjugated format, the antibody permits flexible pairing with user-selected detection chemistries, including HRP, biotin, or fluorophore conjugates.

For handling, aliquoting upon receipt helps limit repeated freeze-thaw exposure, and short-term refrigerated storage preserves activity. For extended storage, freezing at -20 degrees C is recommended. The polyclonal nature of the reagent enables recognition of multiple epitopes on the insulin molecule, which may improve tolerance to minor conformational differences across sample preparations. Each batch is generated through host immunization with purified insulin antigen, yielding high-titer serum suitable for direct application or further affinity purification.