Anti-Insulin Antibody

This rabbit polyclonal primary antibody from Antibodies.com is raised against human insulin and supplied as an unconjugated reagent validated for Western Blot detection of endogenous protein. The antibody recognizes insulin in Human, Mouse, and Rat samples, supporting comparative studies of pancreatic islet biology, beta-cell function, and glucose homeostasis across model organisms.

Format and handling: the reagent is formulated at 1 mg/ml in phosphate buffered saline (PBS) at approximately pH 7.2, with 0.05% sodium azide added as a preservative, and is packaged as a 100 µl vial. Refrigerate upon arrival; protect from light, and avoid freeze-thaw cycles to preserve antibody integrity. Briefly centrifuge vials before pipetting to ensure a homogeneous solution.

Recommended use is Western Blotting of cell and tissue lysates to probe proinsulin and mature insulin expression. Pair this reagent with HRP- or fluorophore-conjugated secondary antibodies for chemiluminescent or fluorescent readout. As a polyclonal reagent, the antibody targets multiple epitopes on the insulin molecule, which can improve signal in denatured, reduced SDS-PAGE workflows.