Anti-IL8 Antibody [I8-60]

Mouse monoclonal antibody clone I8-60 from Antibodies.com, biotinylated for use as a detection reagent in immunoassay workflows targeting human interleukin-8 (IL-8, also called CXCL8). The IgG1 isotype reagent is supplied as a liquid in a 100 µl vial, ready for pairing with streptavidin-HRP, streptavidin-alkaline phosphatase, or streptavidin-fluorophore reporters. Clone I8-60 is reactive with human IL-8 and is validated for ELISA applications, where it serves as either a capture or detection antibody in paired-antibody immunoassays. Investigators studying neutrophil chemotaxis, acute-phase inflammatory responses, sepsis-related biomarkers, chronic inflammatory disease, and tumor microenvironment signaling use biotinylated anti-IL-8 reagents of this kind to quantify low concentrations of the chemokine. The biotin label supports flexibility across detection chemistries: enzyme conjugates for absorbance-based readouts, fluorophores for multiplex bead platforms, or streptavidin-coated plates for capture-orientation assays. Aliquot the reagent upon first thaw and avoid repeated freeze-thaw cycles to preserve biotin integrity. Monoclonal antibodies such as I8-60 deliver epitope-specific recognition that polyclonal preparations cannot replicate, allowing reproducible IL-8 quantitation across runs and between operator hands. The format as a ready-to-use liquid removes the need for reconstitution and allows direct dilution into assay buffers at the bench.