Anti-IL2R Antibody

The Interleukin-2 Receptor alpha chain (IL2Rα, also designated CD25) is a transmembrane glycoprotein expressed on activated CD4+ and CD8+ T lymphocytes, regulatory T cells (Tregs), and certain B cell populations. As the high-affinity binding subunit for IL-2, IL2Rα is central to T cell proliferation, immune tolerance, and the formation of functional immunological synapses. Researchers investigating autoimmunity, transplant rejection, lymphoma biology, and checkpoint immunotherapies routinely use anti-CD25 reagents to track lymphocyte activation states and enumerate FOXP3+ Tregs in tissue sections and cell lysates.

This unconjugated rabbit polyclonal antibody from Antibodies.com is supplied as 100 µl of ready-to-use reagent and is validated for western blot and immunohistochemistry on human, mouse, and rat samples. Its polyclonal format yields recognition of multiple epitopes across the IL2Rα ectodomain, supporting robust signal in formalin-fixed paraffin-embedded (FFPE) sections and standard immunoblot workflows. Common working dilutions span 1:100 to 1:1,000 for IHC and 1:500 to 1:2,000 for western blot, depending on antigen density and detection chemistry.

Suggested applications include characterisation of activated T cell infiltrates in tumour microenvironment studies, phenotyping of CD4+CD25+ Treg populations in models of multiple sclerosis, rheumatoid arthritis, and inflammatory bowel disease, and monitoring IL2Rα expression during allograft rejection research. The unconjugated format allows pairing with user-selected secondary antibodies and flexible label chemistries, including HRP, biotin, or fluorophore conjugates. For handling, refrigerate upon arrival and aliquot to limit freeze-thaw cycles during long-term storage.