Anti-IL2 Antibody

Interleukin-2 (IL-2) is a secreted cytokine that drives proliferation, effector differentiation, and survival of activated T lymphocytes, making it a focal target in studies of immune regulation, autoimmunity, graft rejection, and tumor immunology. This unconjugated rabbit polyclonal reagent from Antibodies.com detects the human cytokine and is supplied as a 100 µl vial, formatted for western blot, immunohistochemistry, and immunofluorescence workflows on human-derived samples and cell-line models.

Raised in rabbit and affinity-purified, the antibody recognizes mature human IL-2 and is reactive on human tissue lysates and stimulated primary cells. In western blot, it resolves the cytokine band from activated peripheral blood mononuclear cells or transfected lines, supporting time-course and dose-response analyses of T-cell activation. The unconjugated format gives immunohistochemistry users freedom over detection chemistry on fixed sections, including tumor and inflamed tissue microenvironments where IL-2-producing cells are sparse. For immunofluorescence, the polyclonal pool supports co-staining with activation or lineage markers in cultured lymphocytes.

Aliquot on receipt and refrigerate at 4 °C for routine short-term use; for extended storage, snap-freeze working volumes and store at -20 °C, avoiding repeated freeze-thaw cycles and protecting from light when paired with fluorescent secondaries. A single 100 µl vial provides enough reagent for dozens of immunoblots or several dozen tissue sections, depending on dilution. The unconjugated, human-specific reactivity profile supports research into cytokine network dissection, checkpoint biology, and characterization of tumor-infiltrating lymphocytes in translational settings.