Anti-IL1B Antibody

Interleukin-1 beta (IL1B) is a pro-inflammatory cytokine central to innate immunity, the NLRP3 inflammasome pathway, and the regulation of fever and leukocyte recruitment. Research use of anti-IL1B reagents supports studies in chronic inflammation, autoimmunity, sepsis biology, and tumor microenvironments, where tracking the pro-form and mature cleavage product distinguishes inflammasome activation state.

This unconjugated rabbit polyclonal antibody from Antibodies.com targets human IL1B and is validated for Western blot and immunohistochemistry workflows on human samples. The reagent is supplied at 100 µl, giving flexibility for direct use as a primary antibody or for custom labeling with reporter enzymes or fluorophores in multiplexed imaging panels. Polyclonal reactivity provides broad epitope coverage across both the 31 kDa pro-IL1B precursor and the p17 mature fragment generated by caspase-1 cleavage, supporting western blot work where distinguishing inflammasome processing in stimulated cell lysates matters.

For immunohistochemistry, the reagent is applied to tissue sections from inflamed and neoplastic samples, where IL1B expression in infiltrating myeloid cells and epithelial compartments informs studies of local inflammatory tone. Aliquot upon first thaw and store at -20 degrees C to limit freeze-thaw cycles, which is standard handling for polyclonal sera in research use only workflows.