Anti-IL10 Antibody [4H2]

Supplied by Antibodies.com as a research-grade primary antibody, the Anti-IL10 Antibody (clone 4H2) is a mouse monoclonal IgG2b reagent offered in unconjugated, lyophilised form. The 500 µg pack size is provided as a freeze-dried preparation buffered in 0.01 M phosphate buffered saline at pH 7.0 and is intended to be reconstituted with double-distilled water to a working concentration that is reconstitution dependent. The antibody was raised against purified recombinant human IL-10, giving it specificity for the human cytokine also known as Interleukin-10, and reactivity has been validated for ELISA applications only. Purity is achieved by Protein G affinity chromatography, and the clone is identified as 4H2 within the monoclonal series. Shipping is performed at 4 °C in insulated packaging; on arrival, the vial should be aliquoted and stored long term at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Because no fluorophore or enzyme is coupled to the immunoglobulin, the reagent is well suited as a capture or detection layer in ELISA plates where a secondary detection system (for example HRP- or biotin-conjugated anti-mouse IgG) will be introduced downstream. The lyophilised format simplifies international logistics, since cold-chain transit is only required during the initial dispatch from St. Louis, MO, after which the product tolerates ambient temperatures during the typical 6–9 business day lead time. Researchers working on cytokine profiling, immune-regulation studies, or anti-inflammatory screening panels in human samples will find the clone a reliable tool, while users planning multiplex or intracellular staining workflows should note that conjugates and additional validated applications are not claimed for this particular lot. The reagent is for research use only and is not intended for diagnostic or therapeutic applications.