Anti-IL-8 Antibody

This rabbit polyclonal reagent from Antibodies.com targets Interleukin-8 (IL-8 / CXCL8), a pro-inflammatory CXC chemokine that drives neutrophil chemotaxis and activation during acute and chronic inflammation. The unconjugated antibody is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, and is validated for western blot and immunohistochemistry on human tissue and cell samples. Each 100 µl vial contains storage-buffer-stabilized liquid ready to dilute for blotting or staining workflows. IL-8 is secreted chiefly by macrophages, endothelial cells, and airway epithelium in response to TNF-alpha, IL-1beta, or microbial products, and elevated expression has been associated with neutrophilic airway disease, sepsis, rheumatoid arthritis, and tumor angiogenesis. The mature chemokine signals through CXCR1 and CXCR2 G-protein-coupled receptors, making it a target of interest in cytokine biology, host-defense research, and oncology studies characterizing the tumor microenvironment. Researchers use this polyclonal antibody to detect endogenous IL-8 in cell lysates and fixed tissue sections; store at -20 degrees C for long-term stability, with freeze-thaw cycles minimized by preparing working aliquots.