Anti-IL-6 Antibody

The IL-6 target is a pro-inflammatory cytokine central to acute-phase signaling, B-cell maturation, and T-cell differentiation. This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit and validated for both Western blot (WB) and ELISA workflows against human samples, making it a versatile tool for cytokine research and inflammation studies.

Each vial contains 100 µl of antibody, supplied as a liquid stock for direct dilution into blocking, wash, or assay buffers. Researchers commonly deploy the reagent to probe IL-6 expression in cell culture supernatants, serum-derived samples, and lysates prepared from stimulated cells. As a polyclonal reagent, the antibody recognizes multiple epitopes across the IL-6 molecule, which can improve signal detection of endogenous protein in Western blot applications.

The unconjugated format allows flexible pairing with user-selected secondary labels such as HRP, biotin, or fluorophores suited to a given detection platform. Store refrigerated for short-term use, or aliquot and freeze at -20 °C for longer-term stability; avoid repeated freeze-thaw cycles to preserve binding activity. Handle the reagent under standard laboratory cold-chain conditions and protect from light when working with downstream conjugates.