Anti-IL-6 Antibody

Interleukin-6 (IL-6) is a pleiotropic cytokine central to inflammatory signaling, acute-phase responses, and immune cell differentiation. This polyclonal reagent from Antibodies.com is raised in rabbit and affinity-purified to recognize the native IL-6 target across human, mouse, and rat sample backgrounds, supporting translational studies that span rodent disease models and clinical specimens.

The antibody is supplied as a 100 µl aliquot at 1 mg/ml in PBS, with 50% glycerol and 0.1% sodium azide added as a stabilizer and preservative. The unconjugated format leaves it ready for custom labeling or direct use in standard immunoassays. Validated applications include Western blot for protein-level verification in cell lysates and tissues, and immunohistochemistry for localizing IL-6 expression in fixed sections.

For best results, store the vial frozen at -20°C in small working aliquots, protect from light, and minimize freeze-thaw cycles to preserve full binding activity. The polyclonal clonality provides recognition of multiple epitopes, which is advantageous when probing IL-6 isoforms, post-translational variants, or truncated fragments. Short-term handling at 4°C is acceptable for daily use.

Researchers commonly deploy this reagent when investigating cytokine storm mechanisms, autoimmune pathogenesis, oncology signaling, and hepatic or neural inflammatory pathways, where reliable species cross-reactivity and batch-to-batch consistency are critical.