Anti-IL-4Rα Antibody

Manufactured by Antibodies.com, this affinity-purified polyclonal reagent targets the alpha subunit of the interleukin-4 receptor (IL-4Rα, also known as CD124) and is supplied in a 50 µl volume at 1 mg/mL in a PBS buffer containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Raised in rabbit against a synthetic peptide derived from the non-phosphorylation site at Y497 of human IL-4Rα, the unconjugated antibody has been validated for ELISA, Western blot and immunocytochemistry/immunofluorescence (ICC/IF) workflows, and it cross-reacts with both human and mouse samples, supporting cross-species studies of cytokine receptor signalling. Specificity has been confirmed by Western blot analysis of 293 cells, where the reagent detects endogenous levels of IL-4Rα protein. The product is shipped at 4 °C; upon arrival it should be aliquoted and stored at -20 °C, with repeated freeze/thaw cycles avoided to preserve antibody integrity. A 100 µl format is also available from the supplier for laboratories with higher demand. Intended strictly for research use and not for diagnostic or therapeutic applications, this Antibodies.com polyclonal offers a reliable and well-characterised option for investigators studying Th2-mediated immune responses, IL-4/IL-13 signalling pathways and related areas of receptor biology.