Anti-IL-27Rα Antibody

Raised in rabbit against the IL-27Rα subunit, this unconjugated polyclonal reagent from Antibodies.com is supplied as a 50 µL liquid at 1 mg/mL in phosphate-buffered saline (PBS) containing0.05% sodium azide at approximately pH 7.2. The antibody is of the IgG isotype and has been validated for Western blot (WB) detection of endogenous IL-27Rα (also known as TCCR, WSX-1, CRL1, or IL-27RA), a type I cytokine receptor subunit that pairs with gp130 to mediate IL-27 signaling. It cross-reacts with human, mouse, and rat samples, making it suitable for comparative studies across these species. The product is shipped on cold packs and should be aliquoted and stored at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. As an affinity-purified polyclonal raised against an IL-27Rα-derived immunogen, it offers broad epitope coverage, which can be advantageous when probing for the full-length receptor in lysates prepared from lymphoid tissues such as spleen and lymph nodes, or from cell lines relevant to Th1-type T-cell and innate immune research. Researchers should empirically optimize dilution for their particular Western blot system; starting points in the 0.5–2 µg/mL range are typical for this class of reagent. For research use only and not intended for diagnostic or therapeutic applications.